Free Biochemistry MCQs Mock Test Online with Answers

Q1 : The immediate precursor of norepinephrine is______________?

A: Epinephrine B: Tyrosine C: Dopamine D: Phenylalanine

Q2 : Inactive precursors of enzymes are known as_______________?

A: Apoenzymes B: Coenzymes C: Proenzymes D: Holoenzymes

Q3 : The main function of mitochondria is______________?

A: Protein synthesis B: Oxidation C: Electron transfer D: Fat synthesis

Q4 : Codons are present on_____________?

A: m-RNA B: DNA C: t-RNA D: Ribosomal RNA

Q5 : Which of the following enzymes use NADP as coenzyme ________________?

A: Glyceraldehyde 3 phosphate dehydrogenase B: Lactate dehydrogenase C: Glucose 6-Phosphate dehydrogenase D: Beta hydroxy acyl CoA dehydrogenase

Q6 : Which of the following process in involved in conversion of DNA to RNA___________?

A: Conjugation B: Transduction C: Translocation D: Transcription

Q7 : Which of the following is not a product of HMP shunt_____________?

A: NADPH B: D fructose 6 phosphate C: D sedoheptulose 5 phosphate D: D glyceraldehyde 3 phosphate

Q8 : In molecular cloning, Blue White screening is used for_______________?

A: To screen for recombinant vectors B: To detect gene mutations C: To identify desired chromosomal DNA insert in plasmid vectors D: To detect host DNA in situ

Q9 : At PH 7 the binding of 2,3 DPG to hemoglobin occurs at which site ?

A: Sulphydryl group B: Carboxy terminal C: Amino terminal D: Histidine

Q10 : The end product of glycolysis under anaerobic conditions is_______________?

A: Lactic acid B: Pyruvic acid C: Acetoacetic acid D: Oxaloacetic acid

Q11 : Alkaptonuria an inherited metabolic disorder is due to the deficiency of____________?

A: Homogentisate oxidase B: Cystathionase C: Pheylalanine hydroxylase D: Tyrosine transaminase

Q12 : Which of the following is the best site of absorption of calcium ?

A: Proximal segment of small intestine B: Middle segment of small intestine C: Ascending colon D: Ileum

Q13 : Ketone bodies are normally synthesized from______________?

A: Acetyl-CoA B: Glucose C: Glycerol D: Acetone

Q14 : Enzyme that catalyses the transfer of a molecule group from one molecule to another is_____________?

A: Oxidases B: Peptidases C: Transferases D: LIpases

Q15 : Adipose tissue releases free fatty acids in the________________?

A: Fasting state B: Resting state C: Active state D: All of the above

Q16 : Dietary fibre is rich in_____________?

A: Starch B: Cellulose C: Collagen D: Inulin

Q17 : Which of the following organs cannot use ketone bodies_______________?

A: Brain B: Liver C: Kidney D: Spleen

Q18 : Coenzyme responsible for single carbon transfer is______________?

A: Acetyl co enzyme A B: Biotin C: THF4 D: Pyridoxine

Q19 : Thyroxine and catecholamines are derived from_____________?

A: Tyrosine B: Tryptophan C: Alanine D: Leucine

Q20 : Lipid is required in the average diet because it________________?

A: has a high caloric value B: Provides essential fatty acid C: aids in absorption of carbohydrates D: is necessary for storage of carbohydrates

Q21 : Non polar lipid act as______________?

A: Electrical insulators B: Irons C: Non electrical insulators D: Electrolytes

Q22 : The key enzyme of gluconeogenesis is______________?

A: Pyruvate carboxylase B: Fructose 1,6 disphosphatase C: Glucose 6 phosphatase D: Phosphoenol pyruvate carboxykinase

Q23 : Which of them is multienzyme complex_____________?

A: Pyruvate dehydrogenase B: Pyruvate dehydrogenase C: Succinate dehydrogenase D: Both A & B

Q24 : On complete hydrolysis of DNA we will get all the following except______________?

A: Deoxy pentose sugar B: Phosphoric acid C: Adenosine D: Purine bases

Q25 : Which of the following is not a second messenger ?

A: c AMP B: c GMP C: Phosphatidyl inosital D: Phosphatidyl glycerol

Q26 : The conversion of glucose 6-P to fructose 6-P is an example of which of the following reactions___________?

A: Phosphate transfer B: Isomerisation C: Dehydration D: Aldol cleavage

Q27 : The lipoprotein particles that have the highest percentage concentration of cholesterol is_______________?

A: Chylomicron B: VLDL (Very low density lipoprotein) C: VLDL (Very low density lipoprotein) D: HDL (High density lipoprotein)

Q28 : The activity of the following enzyme is affected by biotin deficiency____________?

A: Transketolase B: Dehydrogenase C: Oxidase D: Carboxylase

Q29 : In mycocarrdial infarction the first enzyme to rise____________?

A: CPK B: LDH C: SGOT D: Amylase

Q30 : The process by which a base sequence of messenger RNA is synthesized (by a RNA polymerase) on a template of complementary DNA is called______________?

A: Transcription B: Transduction C: Translation D: . Translocation

Q31 : Which of the following is correct of the pyruvate dehydrogenase enzyme complex ?

A: It is present in cytoplasm B: Catalyzes the conversion of pyruvate to oxaloacetate C: Requires TPP as cofactor D: Catalyzes a reversible reaction

Q32 : An example of layses is_____________?

A: Glutamine synthetase B: Fumarase C: Cholinesterase D: Amylase

Q33 : Which one of the following human tissues contains the greatest amount of body glycogen ?

A: Liver B: Kidney C: Skeletal muscle D: Cardiac muscle

Q34 : Triglycerides in serum are transported by_______________?

A: HDL B: VLDL C: LDL D: Chylomicrons

Q35 : Fatty aids help in synthesis of all except____________?

A: Glucose B: Cholesterol C: Ketone bodies D: Fat

Q36 : Increase in lactate and pyruvate is seen in deficiency of_____________?

A: Pyridoxin B: Niacin C: Thiamine D: Retinotic acid

Q37 : During phagocytosis, the metabolic process called respiratory burst involves the activation of_____________?

A: Oxidase B: Hydrolase C: Peroxidase D: Dehydrogenase

Q38 : The major lipids that make up the cell membrane are_______________?

A: Triglycerides B: Phospholipids C: Sphingomyelins D: Fatty acids

Q39 : The daily requirement of protein for the adults is_________________?

A: 6 gms B: 60 gms C: 120 gms D: 250 gms

Q40 : Most importent compansatory mechanism in metabolic acidosis______________?

A: Hyperventilation B: Increased NH3 excretion by kidneys C: Increased urinary phosphates D: Increased HCO3 Production

Q41 : Iron is present in all, EXCEPT______________?

A: Myoglobin B: Cytochrome C: Catalase D: Pyruvate kinase

Q42 : Enzymes concerned with the citric acid cycle are found in the____________?

A: Nucleus B: Ribosomes C: Mitochondria D: Nonparticulate cytoplasm

Q43 : The following is not a phospholipid______________?

A: Sphingomyelin B: Lecithin C: Cerebroside D: . Cephalin

Q44 : Proteins are linear polymers of amino acids, They fold into compact structures Sometimes these folded structures associate to form homo or hetero dimers Which one of the following refers to this associated form ?

A: Denatured state B: Molecular aggregation C: Precipitation D: Quaternary structure

Q45 : The following is not a carrier protein________________?

A: Cerruloplasmin B: Transferrin C: Transcobalamine D: Haptoglobulin

Q46 : Urea is formed in_____________?

A: Brain B: Kidney C: Liver D: intestine

Q47 : The following are derived from cholesterol, EXCEPT_____________?

A: Bile acids B: Steroid hormones C: Vitamin D D: Energy

Q48 : The first product of glycogenolysis is_____________?

A: Glucose 6 phosphatase B: Glucose 1,6 diphosphatase C: Glucose 1- phosphatase D: Fructose 1- phosphatase

Q49 : Heme is converted to bilirubin mainly in_____________?

A: Kidney B: Liver C: Spleen D: Bone marrow

Q50 : Fraction of CPK is an enzyme that is elevated in a myocardial infarct is____________________?

A: MM fraction B: MB fraction C: BB fraction D: Both MM and BB fraction

Q51 : Histidine is converted to histamine by_____________?

A: Transamination B: Hydroxylation C: Hydroxylation D: Reduction

Q52 : The citric acid cycle is the final pathway for oxidation of______________?

A: Enzymes B: Enzymes C: Minerals D: None of the above

Q53 : Ammonia is detoxified in liver to form____________?

A: Uric acid B: Glutamine C: Creatinine D: Urea

Q54 : Free radicals can be inactivated by the following enzymes EXCEPT__________________?

A: Glutathione peroxidase B: Catalase C: Superoxide dismutase D: Myeloperoxidase

Q55 : Acetyl CoA is produced from which fuel sources_____________?

A: Carbohydrate B: Lipid C: Amino acids D: All of the above

Q56 : Which of the following is used in recombinant DNA technology ?

A: Restriction endonucleases B: PCR C: Reverse transcriptase FISH D: None of these

Q57 : The abundant glycoprotein in the basement membrane and has binding domain for both ECM and cell-surface receptor is______________?

A: Laminin B: Fibronectin C: E – Cadherins D: Tenascin

Q58 : Which of the following is most important content of diet ?

A: Protein B: Water C: Vitamin D: Minerals

Q59 : Genes are_____________?

A: Ribonucleic acid B: Deoxy ribonucleic acid C: Lipoproteins D: Chromo proteins

Q60 : HMG-CoA is formed during metabolism of all except______________?

A: Cholesterol B: Leucine C: Ketone bodies D: Bile acids

Q61 : Hyponatremia is defined as a serum Na+ level ____________________?

A: < 135 mmol/L B: . >135 mmol/L C: < 160 mmo/L D: < 100 mmo/L

Q62 : Nonsense codons bring about__________________?

A: Elongation of polypeptide chain B: Pre-translational modification of protein C: Initiation of protein synthesis D: Termination of protein synthesis

Q63 : Cofactor required for lipoprotein lipase activity is_______________?

A: Apo A-II B: Apo C-II C: Apo C-III D: Apo B-II

Q64 : The activity of this plasma enzyme is responsible virtually for all plasma cholesterol esters in humans_______________?

A: Lecithin cholesterol acyl transferase (LCAT) B: Pyrophospho mevalonate decarboxylase C: HMG CoA reductase D: Phospho mevalonate kinase

Q65 : The nitrogen content in 50 gm of a typical dietary protein is most likely to be_____________?

A: 5 gm B: . 8 gm C: 10 gm D: 16 gm

Q66 : In Niemann-Pick’s disease the following substrance accumulate in CNS in excess______________?

A: Glycerophosphatides B: Phosphoinositides C: Phosphosphingosides D: Glycerosphingosides

Q67 : Urinary protein is detected by______________?

A: Barfoed test B: Hay’s test C: Boiling test D: Ehrlich’s test

Q68 : The enzyme that is specifically increased in obstructive jaundice is_____________?

A: Glutamate oxaloacetate transaminase B: Glutamate pyruvate transaminase C: Alkaline phosphaase D: Acid phosphatase

Q69 : Coenzyme, in an enzymatic reaction usually functions to_____________?

A: Activate the substrate B: Increase the active sites of apoenzyme C: Enhance the specificity of apoenzyme D: Accept one of the cleavage products

Q70 : Synthesis of protein occurs on_____________?

A: Mitochondria B: Poly ribosomes C: Nucleus D: Golgi bodies

Q71 : Unwinding of DNA is done by____________?

A: DNAse B: Topo isomerase C: Ligase D: Reverse transcriptase

Q72 : Which acid is formed in the citric acid cycle ?

A: Oxaloacetic acid B: Glutamic acid C: Nitric acid D: None of the above

Q73 : The enzyme phosphofructo kinase-1 is strongly activated by_______________?

A: Cyclic AMP B: Adenosine tri phosphate C: Citrate D: Fructose 2,6 bis phosphate

Q74 : The precursor of steroid is_______________?

A: Amino acids B: Fatty acids C: Cholesterol D: None of the above

Q75 : The best technique useful for initial viral load estimation is________________?

A: Real time PCR B: Widal test C: Electrophoresis D: Immunofluoresence

Q76 : ALbumin is synthesized by_____________?

A: Liver B: Kidney C: Muscle D: Spleen

Q77 : Which of the following is abnormal constituent of urine_____________?

A: glucose B: Creatine C: Urea D: None of the above

Q78 : The sigma subunit of prokaryotic RNA polymerase_______________?

A: Binds the antibiotic Rifampicin B: Is inhibited by a-amanitin C: Specifically recognizes the promoter site D: Is part of the core enzyme

Q79 : HMP shunt is of great importance in cellular metabolism because its produces_____________?

A: ATP B: ADP C: Acetyl CoA D: NADPH

Q80 : The enzyme involved in the first committed step of glycolysis is____________?

A: Phosphofructokinase B: Glucose-6-Phosphatase C: Hexokinase D: Enolase

Q81 : The compound that can give rise to glucose by gluconeogenesis is_____________?

A: Acetyl CoA B: Lactate C: Palmitic acid D: Fructose

Q82 : The number of essential amino acid are______________?

A: 6 B: 8 C: 12 D: 16

Q83 : Which one of the following enzymes provides a link between glycolysis and the citric acid cycle ?

A: Lactate dehydrogenase B: Pyruvate kinase C: Citrate synthase D: Pyruvate dehydrogenase

Q84 : Nor epinephrine is converted to epinephrine by______________?

A: Dopamine hydroxylase B: Dopa decarboxylase C: O-methylation D: N-methylation

Q85 : Somatomedin mediates________________?

A: Deposition of chondroitin sulfate B: Lipolysis C: Gluconeogenesis D: Decreased rate of glucose uptake by cells

Q86 : Normal blood bilirubin level is______________?

A: 0.5 – 1 mg% B: 1 – 3 mg% C: 3 – 4 mg% D: 4 – 5.5 mg%

Q87 : The protein rich in basic amino acids, which functions in the packaging of DNA is chromosomes is______________?

A: Histone B: Collagen C: Hyaluronic acid binding protein D: Fibrinogen

Q88 : Inside the cell, the substance, which contributes to most of the osmolality, is________________?

A: Protein B: Potassium C: Urea D: Phosphate

Q89 : Triglyceride synthesis synthesis is increased by______________?

A: Growth hormone B: Insulin C: Cortisol D: Gulcagon

Q90 : Detoxification of drugs is controlled by_____________?

A: Cytochrome B: Cytochrome P450 C: Cytochrome C D: Cytochrome A

Q91 : In which type of glycogen storage disease is hyper uricemia a feature ?

A: I B: II C: III D: IV

Q92 : Proteins are absorbed from GIT as_____________?

A: Amino acids B: Peptides C: Peptones D: All of the above

Q93 : An enzyme not involved in glycolysis is______________?

A: Enolase B: Phosphoglycero mutase C: Aldolase D: Glycerophosphate dehydrogenase

Q94 : Insulin caused lipogenesis by all except____________?

A: Increasing acetyl-CoA corboxylase activity B: Increases the transport of glucose in to the cells C: Inhibits Pyruvate dehydrogenase D: Decreases intracellular cAMP level

Q95 : Iron is present in all of the following except_______________?

A: Myoglobin B: Cytochrome C: Catalase D: Pyruvate kinase

Q96 : A example of a preventive antioxidant is_______________?

A: Catalase B: Tocopherol C: Superoxide dismutase D: Urate

Q97 : End product of purine metabolism is______________?

A: Creatinine B: Uric acid C: Xanthine D: Phosphates

Q98 : Enzymes increase reaction rates by______________?

A: Altering the free energy of the action B: Inhibiting the backward reaction C: Enhancing the forward reaction D: Decreasing the energy of activation

Q99 : Major contribution towards gluconeogenesis is by____________?

A: Lactate B: Ketones C: Glycerol D: Alanine

Q100 : Prostaglandin synthesis is partially switched off by______________?

A: Lipo oxygenase inhibition B: Cyclo-oxygenase inhibition C: Reductase D: Reductase

Free Biochemistry MCQs Mock Test Online with Answers

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