Free Biochemistry MCQs Mock Test Online with Answers

Q1 : Examples of monounsaturted fatty acids are______________?

A: Oleic acid B: Arachitlonic acid C: Linolenic acid D: Palmitic acid

Q2 : Which one of the following enzymes provides a link between glycolysis and the citric acid cycle ?

A: Lactate dehydrogenase B: Pyruvate kinase C: Citrate synthase D: Pyruvate dehydrogenase

Q3 : McArdles disease is due to the deficiency of_____________?

A: Glucose 1 phosphatase B: Glucose 1,6 phosphatase C: Glucose 6 phosphatase D: Myophosphorylase

Q4 : Glycogen breakdown leads to formation of______________?

A: Glucose B: Lactic acid C: Glucose & Lactic acid D: Glycoprotein

Q5 : Heme is converted to bilirubin mainly in_____________?

A: Kidney B: Liver C: Spleen D: Bone marrow

Q6 : Urea is formed in_____________?

A: Brain B: Kidney C: Liver D: intestine

Q7 : In Hartnup’s disease _______ is excreted in the urine?

A: Ornithine B: Glutamine C: Tryptophan D: Phenylalanine

Q8 : An enzyme that makes a double stranded DNA copy from a single stranded RNA template molecule is known as____________?

A: DNA polymerase B: RNA polymerase C: Reverse transcriptase D: Phospho kinase

Q9 : Synthesis of protein occurs on__________________?

A: Mitochondria B: Poly ribosomes C: Nucleus D: Golgi bodies

Q10 : Inactive precursors of enzymes are known as_______________?

A: Apoenzymes B: Coenzymes C: Proenzymes D: Holoenzymes

Q11 : Which of the following process in involved in conversion of DNA to RNA___________?

A: Conjugation B: Transduction C: Translocation D: Transcription

Q12 : Cholesterol levels in abnormal person should be preferably less than_____________?

A: 200 mg/dl B: 180 mg/dl C: 150 mg/dl D: 120 mg/dl

Q13 : The normal level of serum cholesterol is_______________?

A: 100-140 mgs/100 ml B: 260-360 mgs/100 ml C: 150-250 mgs/100 ml D: 80-250 mgs/100 ml

Q14 : The portion of DNA in Which RNA polymerase binds and starts transcription is called______________?

A: Terminator B: Anti terminator C: Operator D: Promoter region

Q15 : Fatty acid oxidation occurs in______________?

A: Cytoplasm B: Microsomes C: Mitochondria D: All of the above

Q16 : Enzyme + coenzyme constitutes_____________?

A: Apoenzyme B: Proenzyme C: Protoenzyme D: Holoenzyme

Q17 : Number of ATP molecules generated in the conversion of glycogen of lactate is_____________?

A: 2 B: 36 C: 38 D: 32

Q18 : Hydroxylation of proline require the following except____________?

A: Fe+2 B: O2 C: Ascorbic acid D: Succinate

Q19 : In all the following RNA participates directly except________________?

A: Post translation modification B: Post transcriptional modification C: DNA replication D: Splicing

Q20 : Normal serum alkaline phosphatase level is_____________?

A: 100-400 Bodansky units B: 1000-4000 Bodansky units C: 0.1-0.4 Bodansky units D: 1-4 Bodansky units

Q21 : One of the earliest symptoms of Vit. A deficiency is________________?

A: Nyctalopia B: Xerophthalmia C: Bitot’s spot D: Keratomalacia

Q22 : Restriction endonuclease cleaves______________?

A: Double stranded DNA B: SIngle stranded DNA C: SIngle stranded RNA D: Polypeptide

Q23 : Iron absorption is decreased in presence of all except____________?

A: Phytates B: Ascorbic acid C: Tannins D: Phosphates

Q24 : The protein rich in basic amino acids, which functions in the packaging of DNA is chromosomes is______________?

A: Histone B: Collagen C: Hyaluronic acid binding protein D: Fibrinogen

Q25 : DNA double helix is bound by_____________?

A: Covalent bond B: Hydrogen bond C: Disulfide linkage D: Vander wall forces

Q26 : Enzymes are classified according to_________________?

A: Action on substrate B: Action of coenzymes C: Nature of the enzymes D: All of the above

Q27 : The main function of mitochondria is______________?

A: Protein synthesis B: Oxidation C: Electron transfer D: Fat synthesis

Q28 : The most important role of cholesterol is_____________?

A: It is a component of cell membrane B: It is a precursor of polyunsaturated fatty acids C: It stores up energy D: None of the above

Q29 : The most important buffer in RBC is____________?

A: O2 Hb +Na hemoglobinate B: O2Hb +K hemoglobinate C: H2CO3 + KHCO3 D: H2CO3 +NAHCO3

Q30 : Generation of free radicals occurs by all of the following mechanism EXCEPT_____________?

A: Normal metabolism B: Oxygen toxicity C: Reperfusion after ischemic injury D: All of the above

Q31 : FSH, LH, TSH acts through_________________?

A: Cyclic AMP B: Cyclic GMP C: Adenosine cyclate D: Calcium release

Q32 : Translation occurs at______________?

A: Mitochondria B: Centrosome C: Nucleus D: Ribosome

Q33 : Serum creatine kinase-3 (CK-3) is elevated in_______________?

A: Muscular dystrophy B: Myocardial infarction C: Alcoholic cirrhosis D: Brain tumours

Q34 : Which of the following amino acid is involved in gluconeogenesis______________?

A: Glycine B: Valine C: Cysteline D: All of the above

Q35 : Ketone body formation takes place in_______________?

A: Liver B: Kidney C: Spleen D: Blood

Q36 : Iron is present in all, EXCEPT______________?

A: Myoglobin B: Cytochrome C: Catalase D: Pyruvate kinase

Q37 : Blood glucose level cannot be augmented by mobilization of muscle glycogen due to lack of_____________?

A: G-6-P dehydrogenase B: G-6-P phydrogenase C: Aldolase D: Glucokinase

Q38 : Urea is produced by the enzyme_____________?

A: Urease B: Uricase C: Arginase D: Glutaminase

Q39 : All are true about glutathione except ?

A: It conjugates xenobiotics B: It is a tripetide C: It converts hemoglobin to methemoglobin D: It scavenges free radicals and superoxide ions

Q40 : Key glycolytic enzymes_____________?

A: Phosphofructokinase B: Hexokinase C: Pyruvate kinase D: Glucose 1,6 diphosphatase

Q41 : The nitrogen content in 50 gm of a typical dietary protein is most likely to be_____________?

A: 5 gm B: . 8 gm C: 10 gm D: 16 gm

Q42 : Which of the following is a non-reducing sugar____________?

A: Glucose B: Maltose C: Lactose D: Sucrose

Q43 : Detoxification of drugs is controlled by_____________?

A: Cytochrome B: Cytochrome P450 C: Cytochrome C D: Cytochrome A

Q44 : The normal value of serum potassium level is____________?

A: 2.8-3.8 meq/L B: 3.8-5 meq/L C: 5-5.8 meq/L D: 6-7.2 meq/L

Q45 : Cell membrane consists of_________________?

A: Lipids and proteins B: Lipids only C: Protein only D: None of the above

Q46 : When the prosthetic group of an enzyme is an inorganic ion, it is called a_____________?

A: Cofactor B: Coenzyme C: Apoenzyme D: Holoenzyme

Q47 : Which of the following is not a ketone body______________?

A: Acetone B: Acetic acid C: Acetocetate D: Beta hydroxybutyric acid

Q48 : Triglyceride synthesis synthesis is increased by______________?

A: Growth hormone B: Insulin C: Cortisol D: Gulcagon

Q49 : Fat provides ______% of body’s calories?

A: 40% B: 30% C: 50% D: 75%

Q50 : The end product of glycolysis under anaerobic conditions is_______________?

A: Lactic acid B: Pyruvic acid C: Acetoacetic acid D: Oxaloacetic acid

Q51 : Ammonia is detoxified in liver to form____________?

A: Uric acid B: Glutamine C: Creatinine D: Urea

Q52 : Which of them is multienzyme complex_____________?

A: Pyruvate dehydrogenase B: Pyruvate dehydrogenase C: Succinate dehydrogenase D: Both A & B

Q53 : Michaelis-Menten hypothesis states that_____________?

A: Rate of enzymatic reaction is independent of substrate concentration B: Rate of enzymatic reaction is independent of substrate concentration C: Km is the enzyme substrate complex association constant D: Enzyme- substrate complex formation is essential in enzymatic reaction

Q54 : Most common non protein nitrogenous fraction of blood_____________?

A: Urea B: Uric acid C: Urobilinogen D: Creatinin

Q55 : Coenzyme responsible for single carbon transfer is______________?

A: Acetyl co enzyme A B: Biotin C: THF4 D: Pyridoxine

Q56 : The daily requirement of protein for the adults is_________________?

A: 6 gms B: 60 gms C: 120 gms D: 250 gms

Q57 : HMG-CoA is formed during metabolism of all except______________?

A: Cholesterol B: Leucine C: Ketone bodies D: Bile acids

Q58 : Cane sugar is____________?

A: Glucose B: Surose C: Fructose D: Maltose

Q59 : Proteins are linear polymers of amino acids, They fold into compact structures Sometimes these folded structures associate to form homo or hetero dimers Which one of the following refers to this associated form ?

A: Denatured state B: Molecular aggregation C: Precipitation D: Quaternary structure

Q60 : Heme in haemoglobin is____________?

A: Between Helix C and D B: Surrounded by non polar environment C: Bonded to E7 histidine D: Protoporophyrin IX

Q61 : Which one of the following is a monosaccharide______________?

A: Maltose B: Sucrose C: Fructose D: Strach

Q62 : Ubiqultin is involved in_____________?

A: Intracellular proteolysis B: Electron transport chain C: Transport of ATP D: Protein folding

Q63 : Free radicals are chemical species which have______________?

A: Single unpaired electron in an outer orbit B: Single paired electron in an outer orbit C: Double unpaired electron in an outer orbit D: Double paired electron in an outer orbit

Q64 : Endogenous triglycerides in plasma is maximally bound to________________?

A: Chylomicrons B: LDL C: VLDL D: HDL

Q65 : Iron is complexed in haemoglobin to_______________?

A: Leucine B: Histidine C: Isoleucine D: Valine

Q66 : Multiienzyme complex in human_______________?

A: Fatty acid synthetase B: Malonyl CoA carboxylase C: Carbomoyl Phosphate synthetase D: Adenosine phospho ribosyl transferase

Q67 : Which of the following is most important content of diet ?

A: Protein B: Water C: Vitamin D: Minerals

Q68 : Which of the following is the best site of absorption of calcium ?

A: Proximal segment of small intestine B: Middle segment of small intestine C: Ascending colon D: Ileum

Q69 : Codons are present on_____________?

A: m-RNA B: DNA C: t-RNA D: Ribosomal RNA

Q70 : Which of the following is precursor of adrenaline and thyroxine synthesis ?

A: Phenyl alanine B: Tyrosine C: Tryptophan D: None of the above

Q71 : In ECF main electrolyte is_______________?

A: Na+ B: K+ C: Cl D: Proteins

Q72 : The process by which a base sequence of messenger RNA is synthesized (by a RNA polymerase) on a template of complementary DNA is called______________?

A: Transcription B: Transduction C: Translation D: . Translocation

Q73 : Which is the poorest source of calcium among the following______________?

A: Rice B: Human milk C: Egg yolk D: Cow’s milk

Q74 : Bile acids are formed from__________?

A: Cholesterol B: Aminoacids C: Bilirubin D: Lipoproteins

Q75 : Which metabolite of TCA cycle is used in detoxification of ammonia in brain_____________?

A: Which metabolite of TCA cycle is used in detoxification of ammonia in brain_____________? B: C: D:

Q76 : Which of the organelles contain DNA_________________?

A: Nucleus B: Golgi apparatus C: Nucleolus D: Golgi apparatus

Q77 : Enzymes increase reaction rates by______________?

A: Altering the free energy of the action B: Inhibiting the backward reaction C: Enhancing the forward reaction D: Decreasing the energy of activation

Q78 : Which of these is not included in parenteral therapy ?

A: Carbohydrates B: Fibres C: Fats D: Micronutrients

Q79 : Normal level of serum phosphorous is_____________?

A: 2.5 – 4.5 mgm% B: 7-9 mgm% C: 40-50 mgm% D: 1-2 mgm%

Q80 : Which of the following amino acids is quickly converted to tyrosine ?

A: Arginine B: Glycine C: Phenylalanine D: Leucine

Q81 : At PH 7 the binding of 2,3 DPG to hemoglobin occurs at which site ?

A: Sulphydryl group B: Carboxy terminal C: Amino terminal D: Histidine

Q82 : Which of the following is correct about breakdown of hemoglobin (Hb)_____________?

A: Hb→Heme→bilirubin→Urobilinogen B: Heme→Hb→Biliverdin→Urobilinogen C: Hb→Heme→Bilverdin→Uro bilinogen D: Hb→Heme→bilirubin→Urobilinogen→Biliverdin

Q83 : The enzyme that is specifically increased in obstructive jaundice is_____________?

A: Glutamate oxaloacetate transaminase B: Glutamate pyruvate transaminase C: Alkaline phosphaase D: Acid phosphatase

Q84 : Dehydrogenase used as coenzymes all of the following, except________________?

A: FMN B: FAD C: NADP+ D: Ferro protoporphyrin

Q85 : Which of the following has a ketogenic effect on livers______________?

A: Insulin B: Glucagon C: GH D: Androgen

Q86 : Proteins are absorbed from GIT as_____________?

A: Amino acids B: Peptides C: Peptones D: All of the above

Q87 : Enzymes help by____________?

A: Lowering the activation energy B: Increasing the substrate concentration C: Decreasing the surface tension D: Increasing the activation energy

Q88 : Free radicals can be inactivated by the following enzymes EXCEPT__________________?

A: Glutathione peroxidase B: Catalase C: Superoxide dismutase D: Myeloperoxidase

Q89 : NADPH is generated by the action of____________?

A: Glucose 6 phosphate dehydrogenase B: Glucose 1 phosphate dehydrogenase C: Glucose 1,6 diphosphate dehydrogenase D: All of the above

Q90 : Mannose 6 phosphate containing freshly synthesized proteins are directed to_____________?

A: Nucleus B: Lysosomes C: Mitochondria D: Golgi apparatus

Q91 : The key enzyme of gluconeogenesis is______________?

A: Pyruvate carboxylase B: Fructose 1,6 disphosphatase C: Glucose 6 phosphatase D: Phosphoenol pyruvate carboxykinase

Q92 : Acetyl Co-A acts as a substrate for all the enzymes except______________?

A: HMG-Co A synthase B: Malic enzyme C: Malonyl CoA synthetase D: Fatty acid synthetase

Q93 : Which of the following is NOT a characteristic feature of allosteric enzymes ?

A: They are multienzyme complex B: Follow Michaelis-Menton Kinetics C: Presence of Modulator site D: Give sigmoid shaped curve

Q94 : If bile acid recycling time is short, then bile salt reservoir pool will be______________?

A: Increased B: Decreased C: Uchanged D: Has no effect

Q95 : The main site of fluoride inhibition in the Embden Meyerhof pathway of acid____________?

A: ATPase B: Enolase C: Pyruvate Kinase D: Fructose 6 phosphatase

Q96 : Major source of ammonia in the kidney is___________?

A: Urea Aspartate B: Aspartate C: Glutamine D: Glutamate

Q97 : The abundant glycoprotein in the basement membrane and has binding domain for both ECM and cell-surface receptor is______________?

A: Laminin B: Fibronectin C: E – Cadherins D: Tenascin

Q98 : Which acid is formed in the citric acid cycle ?

A: Oxaloacetic acid B: Glutamic acid C: Nitric acid D: None of the above

Q99 : The total blood concentration of ketone bodies in well fed individual is__________?

A: 1 mg% B: 5 mg% C: 12 mg% D: 20 mg%

Q100 : The uptake of glucose by the liver increase following a carbohydrate meal because____________?

A: There is increase in phosphorylation of glucose by glucokinase B: GLUT-2 stimulated by insulin C: Glucokinase has a low Km for glucose D: Hexokinase in liver has a high affinity for glucose

Free Biochemistry MCQs Mock Test Online with Answers

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